The novel combination of sulforaphane and maitake mushroom extract suppresses the expression of matrix metalloproteinases in THP-1 human monocyte/macrophage cells
Category: Inflammation & Cancer, Inflammation & Cancer
Conference Year: 2018
Purpose: Matrix metalloproteinases (MMPs) are proteolytic enzymes that have been associated with the progression of cancer. MMPs are most notable for their role in the degradation of the extracellular matrix allowing for migration, invasion and metastasis but also play a role in the immune system by potentiating cytokine and chemokine activity. Here, we examine the effect of a novel combination of sulforaphane and maitake mushroom extract on the gene expression of several MMPS including MMP-1, -2, -3, -9 and -13. Sulforaphane is a phytochemical produced by the hydrolysis of its precursor glucoraphanin by myrosinase enzyme, both of which are found in cruciferous vegetables like broccoli. Sulforaphane has been shown to positively modulate the Keap1/Nrf2 detoxification and antioxidative system, the heat shock response, and the NF-kB inflammatory pathway. Maitake mushrooms have been used for centuries for their immunomodulatory properties attributed to their beta-glucan content. Methods and Results: THP-1 human monocyte/macrophage cells were treated with LPS (1 ng/mL) with or without the combination of maitake mushroom extract (250 µg/mL) and a physiological level of sulforaphane (0.5 µM) in order to examine its effect on MMP-1,-2,-3, -9 and -13 gene expression via quantitative RT-PCR. The combination was able to significantly suppress the gene expression of MMP-1, -3, -9 and -13, while a trend was observed in suppressing MMP-2. Conclusion: This study demonstrates the effect the combination of sulforaphane and maitake mushroom extract has on reducing the expression of several MMPs involved in the progression of cancer. Previously, we showed the combination of sulforaphane and maitake mushroom extract possesses cytoprotective properties as it induces the expression of detoxification and anti-oxidant genes via the Keap1/Nrf2 pathway as well as diminishes expression of pro-inflammatory mediators. This data further demonstrates that the combination of sulforaphane and maitake mushroom extract may offer additional protective properties by regulating the gene expression of MMPs implicated in cancer progression.
Keywords: Sulforaphane, beta- glucans, matrix metalloproteinases