ASPO Abstracts
Isolation of cottonseed extract that affects human cancer cell growth
Category: Inflammation & Cancer, Lifestyles Behavior, Energy Balance & Chemoprevention
Conference Year: 2018
Abstract Body:
Cottonseeds are classified as either glanded or glandless seeds depending on the presence or absence of yellow pigment glands which contain toxic gossypol, the best studied bioactive component in cottonseeds. Glanded cottonseeds rich in gossypol may have anticancer property and glandless cottonseeds essentially free of gossypol may cause cancer in animal studies. Therefore, it is important to investigate the effect of bioactive components from cottonseeds on cancer cells. The objective of this study was to isolate ethanol extracts from glanded and glandless cottonseeds and investigate their effects on human cancer cells. Glanded and glandless cottonseeds were fractionated into seed coat and kernel fractions followed by removal of oils with chloroform and hexane extractions. Ethanol extracts were isolated from the defatted seed coat and kernel and used to treat cultured human cancer cells derived from breast (MCF7), colon (COLO205), lung (A549) and pancreas (MIA PaCA-2). Cell viability was determined by MTT assay. The yield of ethanol extract was approximately 4% from glanded cottonseed and 2% from glandless cottonseed. Breast cancer cell viability was increased 20-60% after 2-h treatment but decreased 15-25% after 24-h treatment by ethanol extracts from glanded cottonseed extracts. Breast cancer cell viability was decreased 40% after 2-h treatment by glandless seed kernel extract only. Colon cancer cell viability was decreased 10-20% by 2-h treatment but increased 10-30% by 24-h treatment of ethanol extracts. Lung cancer cell viability was decreased 20-40% by kernel extract but not by coat extract. Pancreatic cancer cell viability was mostly increased up to 50% by coat extract but decreased up to 50% by kernel extract. These results suggest that ethanol extracts from cottonseeds contain pro- and anticancer activities. It is important to identify the bioactive components in cottonseed extracts in future studies.
Keywords: Cancer, c cottonseed, e ethanol extract, cell viability