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Real-time PCR Total RNA was extracted with TRIzol and reverse transcribed to cDNA as directed in the RT-PCR Kit (ThermoFisher Scientific, Waltham, MA, USA) used here

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To evaluate the information content generated by the sequence design process, we calculated the entropy across each set of designed sequences, and the relative entropy (Kullback–Leibler divergence, KL) between the distribution of amino acids at each sequence position estimated for the model-designed samples, and the training data