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After a 24 h 100 nM incretin-based peptide pre-treatment and a 24 h 1 ng/mL LPS pro-inflammatory stimulus, cells were harvested using a cell scrape, collected by centrifugation, and re-suspended in RIPA buffer (R0278, Sigma-Aldrich) with 1X Halt TM Protease & Phosphatase Single-Use Inhibitor Cocktail (78442, ThermoFisher Scientific, Waltham, MA, USA) for lysis
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