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Extracellular acidification rate (ECAR) was measured using the Agilent Seahorse Xfe24 analyzer, according to manufacturer instructions for the Glycolysis Stress Test

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Using illumination with a 175-W xenon arc lamp, excitation wavelengths (340/380 nm) were selected via a Lambda DG-4 monochromator wavelength changer (Shutter Instrument, Novato, CA)

1 support with a 321% natural GLP